Abstract
Dipeptides were derivatized with phenyl thiocarbamyl (PITC) to sulfur‐containing compounds. The reaction was performed directly on the chromatographic plate in normal phase chromatography. The detection system was based on an iodine‐azide reaction that was induced by sulfur compounds. The procedure involved spraying the developed plate with sodium azide and starch solution and, subsequently, exposing the plate to iodine vapor. Due to the catalytic effect of C˭S bonds, white spots appeared on the violet‐gray background. The method was found to be competitive with the other detection techniques and its sensitivity range was as low as pmol per spot.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.