Abstract
IODINE has been used as a colour reagent for paper chromatography of amino-acids and their derivatives1,2, imidazoles3, and other nitrogen-containing compounds1,4. Steroids are also revealed by this technique, which is sufficiently sensitive to enable location of 0.5 µgm. œstrone5. The method has not gained wide acceptance possibly only because it cannot be relied on to yield a colour diagnostic of a class of compounds—some nitrogen-containing compounds are not revealed—but its application is simple, and if unsuccessful, other colour reagents can be applied to the same chromatogram. In the most convenient procedure, the dried paper is exposed to iodine vapour (supplied by a few iodine crystals in a closed battery jar) for 10–30 sec.; or the paper may be sprayed with a solution of iodine in carbon tetrachloride3, or may be dipped into a saturated solution of iodine in petroleum ether (b.p. 60°–80°)5. Compounds are revealed usually as brown spots, which fade rapidly.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
