Iodinated amino acid chromatography on polystyrene resins

Abstract

The iodinated amino acids have been separated by elution analysis on ion exchange columns of Dowex 50 × 4 using an automated procedure which presents the radioactivity and iodide determinations on the effluent on a recorder chart. The iodinated amino acids are eluted using ammonium formate buffers containing 30% ethanol. The method is applicable to samples containing iodinated amino acids of concentrations as found in human plasma without carrier and quantitative recovery of I 131 has been achieved.