Abstract
The present study was undertaken to investigate the expression of αvβ3 and αvβ5 integrin-like vitronectin receptors (VNRs) on Candida albicans germ tube and their involvement in its adhesion to vitronectin (VN) and human endothelial cells. By immunofluorescence and FACS analysis, several monoclonal antibodies directed against human αv or β3 integrin subunit or αvβ3 and αvβ5 heterodimers, positively stained C. albicans germ tubes. C. albicans germ tubes specifically adhered (45–50%) to VN and this adhesion was markedly inhibited by RGD-, but not RGE-containing peptides. Adhesion of C. albicans germ tubes to VN was strongly inhibited by anti-αv, anti-β3 or anti-αvβ3, but not by αvβ5 monoclonal antibody. C. albicans germ tube adhesion to VN was also inhibited by glycosaminoglycans (GAGs) such as heparin or chondroitin sulphate. Finally, we show thatC. albicans germ tubes adhere to the human EA.hy 926 endothelial cell line. This adhesion is markedly blocked by anti-β3 monoclonal antibody, GRGDSP peptide or heparin, and is completely abolished by their combination. Overall these results indicate that C. albicans germ tube adherence to VN and to a human endothelial cell line is mediated by αvβ3, but not by αvβ5-like integrin, and depends on GAGs which may act by regulating αvβ3 integrin-like/VN adhesive interaction.
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