Involvement of TRPC7-AS1 Expression in Hepatitis B Virus-Related Hepatocellular Carcinoma.

Shaoliang Zhu,Weiru Huang,Ningfu Peng,Yingyang Liao,Hang Ye,Xiaojie Xu,Ziyu Peng,Muhammad Wasim Khan

doi:10.1155/2021/8114327

Abstract

Objective To investigate the expression of transient receptor potential (TRP) superfamily genes, especially TRPC7-AS1 in hepatitis B virus- (HBV-) related hepatocellular carcinoma (HCC). Methods Three cancer samples of HBV-related HCC at phase IV and matched paracancerous liver tissues were included in the study. Total RNA was extracted, and differential expression of RNA was screened by high-throughput transcriptome sequencing. The expression of TRPC7-AS1 was detected by quantitative real-time PCR. The N6-adenosyl methylation RNA in MHCC97H, HepG2, and HL-7702 was enriched by coimmunoprecipitation with m6A antibody, and the relative level of N6-adenosyl methylation RNA in TRPC7-AS1 was detected. Results The expression of TRP family genes in cancer tissues was higher than that in paracancerous liver tissues, including TRPC7-AS1, TRPC4AP, PKD1P6, and PKD1P1. Moreover, the expression level of TRPC7-AS1 in MHCC97H and HepG2 was also significantly higher than that in L02, a normal liver cell. The methylation level of N6-adenosine of TRPC7-AS1 was lower in HepG2 cells than that in L02 cells. Conclusion TRP superfamily genes, especially TRPC7-AS1, were highly expressed in HBV-related HCC. TRPC7-AS1 could be a potential therapeutic target or diagnostic marker for HCC.

Highlights

Transient receptor potential (TRP) ion channel is a transmembrane protein, which plays key roles in mechanical injury, pain, temperature perception, and osmotic pressure perception by changing cell membrane potential or intracellular calcium concentration [1,2,3,4]
The TRP ion channel family genes in mammals can be divided into six subgroups: TRP canonical (TRPC), TRP vanilloid (TRPV), TRP melastatin (TRPV), TRP ankyrin (TRPA), TRP mucolipin (TRPML), and TRP polycrystalline (TRPP)
If TRP ion channel family genes were not detected in 4 or more samples, those genes are not shown in Figure 1, i.e., TRPC7 was not detected in 6 samples. e expression of TRP ion channel family genes in cancer tissue was higher than those in paracancerous tissue, and the expressions of TRPV6, TRPM4, TRPC1, and PKD1P1 were significantly upregulated compared with those in paracancerous tissues

Summary

Introduction

Transient receptor potential (TRP) ion channel is a transmembrane protein, which plays key roles in mechanical injury, pain, temperature perception, and osmotic pressure perception by changing cell membrane potential or intracellular calcium concentration [1,2,3,4]. The TRP ion channel family genes in mammals can be divided into six subgroups: TRP canonical (TRPC), TRP vanilloid (TRPV), TRP melastatin (TRPV), TRP ankyrin (TRPA), TRP mucolipin (TRPML), and TRP polycrystalline (TRPP). It has been reported that the dysfunction of the TRP ion channel (TRPV4, TRPV1, TRPM4, and TRPM) is considered to be related to obesity or diabetes, and these disorders are related to appetite, insulin secretion, and autoimmune response [6,7,8,9,10]. E downstream of the TRP ion channel family has a function in cell proliferation and is considered to be related to cancer development (ref ). The involvement of TRP family genes in hepatocellular carcinoma (HCC) is still rarely reported

Methods

Results

Conclusion