Involvement of the Oct-1 and AP-2 elements in the transcriptional regulation of the human hematopoietic prostaglandin D synthase gene in megakaryoblastic Dami cells

Abstract

The human hematopoietic prostaglandin D synthase (H-PGDS) gene is expressed in megakaryoblastic cells, and its expression is enhanced by phorbol ester. We analyzed the promoter activity of the 5′-flanking region and the untranslated exon 1 (−1044 to +290) of the human H-PGDS gene in human megakaryoblastic Dami cells. Both promoter-luciferase reporter and electrophoretic mobility shift assays demonstrated that the Oct-1 element in the 5′-flanking region was required for the cell type-specific activation of the H-PGDS gene. The AP-2 element within the untranslated exon 1 was shown to be critical for the basal expression and the phorbol ester-mediated up-regulation of the gene. The H-PGDS promoter activity was effectively enhanced by co-expression with AP-2α in AP-2-deficient HepG2 cells.