Involvement of T-cell-Ig-mucin-3 (Tim-3) in macrophage polarization

Abstract

Event Abstract Back to Event Involvement of T-cell-Ig-mucin-3 (Tim-3) in macrophage polarization Gencheng Han1*, Guojiang Chen1, Beifen Shen1 and Yan Li1 1 Beijing Institute of Basic Medcal Sciences, China T-cell-Ig-mucin-3 (Tim-3) is widely involved in the negative regulation of adaptive immunity, and its dysregulation on CD4+T and CD8+T cells is associated with autoimmune diseases, chronic virus infection or tumor. However, its roles in innate immunity remain unclear. We previously reported that Tim-3 promotes macrophage homeostasis by negatively regulating TLRs response (J.Immunol.2013), indicating that Tim-3 might also negatively regulate innate immunity. Here we further identified a dysregulated downregulation of Tim-3 on lamina propria macrophage in mice with ulcerative colitis (UC), which is related to a M1 shift of macrophage as marked by increased pro-inflammatory cytokines expression including IL-12, IL-6, TNF-a, and IL-1b. Blockade of Tim-3 pathway in vivo using Tim-3 antibody or soluble protein lead to enhanced the shift of M1 macrophage and exacerbated inflammation in UC. On the other hand, in mice with colonic tumor, lamina propria macrophage showed increased Tim-3 expression and increased M2-related anti-inflammatory cytokines including IL-10, TGF-b expression by macrophage. These data indicated that Tim-3 expression is closely related to the polarization of macrophage and its dysregulation significantly affects diseases progression. When the underlying mechanisms were investigated, our data showed that Tim-3 down regulation leads to increased NOS2 expression and decreased Arg-1 and Dectin-1 expression in macrophage and changed micro RNA profiles including increased mi-155, miR-27a expression. The intracellular pathways of Tim-3 mediated microphage polarization were also investigated. A better understanding of this pathway might shed new light on the mechanisms of Tim-3 regulated innate immunity. Keywords: TIM-3, macrophage, polarization vision, autoimmune disease, Colitis associated cancer Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Han G, Chen G, Shen B and Li Y (2013). Involvement of T-cell-Ig-mucin-3 (Tim-3) in macrophage polarization. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00558 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 01 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Gencheng Han, Beijing Institute of Basic Medcal Sciences, Beijing, China, genchenghan@163.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Gencheng Han Guojiang Chen Beifen Shen Yan Li Google Gencheng Han Guojiang Chen Beifen Shen Yan Li Google Scholar Gencheng Han Guojiang Chen Beifen Shen Yan Li PubMed Gencheng Han Guojiang Chen Beifen Shen Yan Li Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.