Abstract
Poly(ADP-ribose) metabolism during differentiation of HL-60 human promyelocytic leukemia cells induced by dimethylsulfoxide was studied. Endogenous poly(ADP-ribose) decreased to 15% of the uninduced level at 14 hours after addition of dimethylsulfoxide, when the morphology, phagocytic activity and DNA synthesis of the cells had not yet changed and myeloperoxidase activity had decreased only slightly. Endogenous poly(ADP-ribose) remained at a low level until day 3, but increased sharply to 800% of the uninduced level on day 5 when most of the differentiated phenotypes became obvious. This is the first report of measurement of the endogenous level of poly(ADP-ribose) during cellular differentiation.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
