Abstract
It is well established that corticotropin-releasing hormone (CRH) is a principal neuropeptide which mediates the adrenocorticotropic hormone (ACTH) secretory response to interleukin (IL)-1 in the rat. It has recently been suggested that besides CRH, arginine vasopressin may also play a stimulatory role in IL-1 induced ACTH secretion. However, it remains to be elucidated whether other neuropeptides possessing an ACTH-releasing activity are involved in this neuroendocrine event. Therefore, in this study, we examined possible roles for oxytocin (OT) and cholecystokinin (CCK)-8 in the IL-1-induced ACTH response, utilizing the technique of immunoneutralization of these peptides. For comparison, we examined the effect of CRH immunoneutralization as well. Human recombinant IL-1 beta (50 ng) was given intracerebroventricularly (to the 3rd ventricle) to freely moving male rats 15 min after injecting specific antiserum against CRH, OT, or CCK-8, or normal rabbit serum (control) via the same route. As expected, anti-CRH antibody significantly suppressed the ACTH response to IL-1 beta. Interestingly, anti-OT antibody acted in the same manner, whereas anti-CCK-8 antibody did not. These results suggest that in addition to CRH and arginine vasopressin, OT may also play a significant role in mediating the IL-1 beta-induced ACTH secretion in the rat.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
