Abstract
AbstractThis chapter studied the changes in the level of reactive oxygen species, activities of enzymes involved in reactive oxygen species scavenging and the level of abscisic acid (ABA) under conditions of CN-mediated elimination of embryonic dormancy in apple cv. Antonówka. Isolated embryos from apple were treated for 6 h with 1 mM HCN. Pretreatment with HCN resulted in increased germination reaching 85% after 7 days, with normal seedling development. HCN increased ABA content in embryos. The level of hydrogen peroxide peaked on the first day of culture in both control and pretreated embryos but was 3-fold greater in pretreated ones, indicating an oxidative stress induction by HCN. Superoxide dismutase activity was maximum both in the control and pretreated embryos during the first day of culture. Glutathione reductase was 2-fold higher in pretreated embryos.
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