Abstract
Arrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as “cipó-una”, it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10–100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief.
Highlights
The study of plants used for medicinal purposes accompanied and promoted the discoveries and development of pure drug substances and, new therapeutics. medicinal plants have been explored for an extensive period, they still present themselves as a remarkable source of new bioactive substances with therapeutic potential [1,2].Brazilian flora is distributed in extremely diverse ecosystems
We propose to investigate the antinociceptive effect of this dichloromethane fraction of A. brachypoda, which has mainly unusual dimeric flavonoids in phytochemical compounds
Arrabidaea brachypoda (DC) Bureau is a medicinal plant that is popularly used in Brazil as a natural therapeutic agent to treat pain and inflammation
Summary
The study of plants used for medicinal purposes accompanied and promoted the discoveries and development of pure drug substances and, new therapeutics. Brazilian flora is distributed in extremely diverse ecosystems Contributing to this diversity, the Cerrado (neotropical savanna) extends mainly over the northeast, middle west, and southeast of Brazil and contains more than 12,000 plant species [3]. The compound(s) responsible for this antinociceptive effect, or even the underlying mechanisms of action whereby this medicinal plant reduces pain, are not well understood. Our previous study with the dichloromethane (DCM) fraction of the roots revealed that these three unusual dimeric flavonoids isolated from this fraction present high in vitro and in vivo activities against Trypanosoma cruzi, the parasite responsible for the Chagas disease [7]. We propose to investigate the antinociceptive effect of this dichloromethane fraction of A. brachypoda, which has mainly unusual dimeric flavonoids in phytochemical compounds. This study will determine the antinociceptive mechanism(s) of action of this medicinal plant
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