Involvement of Nitric Oxide in Methyl Jasmonate-Mediated Regulation of Water Metabolism in Wheat Plants under Drought Stress

Abstract

Drought is a serious challenge that causes significant crop loss worldwide. The developmental processes of plants are regulated by phytohormones and signaling molecules that crosstalk together in signaling cascades. We suppose that nitric oxide (NO) is a secondary messenger of the JAs signaling pathway, as 10−7 M methyl jasmonate (MeJA) pretreatment regulates NO accumulation in wheat plants under drought stress, modulated by 12% polyethylene glycol (PEG), and in control plants. This study aimed to compare 2 × 10−4 M nitric oxide donor sodium nitroprusside (SNP) and MeJA pretreatments in regulating growth and water balance parameters at the vulnerable initial first-leaf stage of wheat growth. The application of 12% PEG decreased transpiration intensity twofold, relative water content (RWC) by 7–9%, and osmotic potential of cell sap by 33–40% compared with those of control plants. Under drought, MeJA- and SNP-pretreated plants decreased transpiration intensity by 20–25%, RWC by 3–4%, and osmotic potential of cell sap by 16–21% compared with those of control plants, and enhanced the proline content by 25–55% compared with MeJA- and SNP-untreated plants. Our results suggest that pretreatment with MeJA as well as SNP could mitigate drought stress in wheat plants. Similarities in MeJA- and SNP-induced shifts in plant water balance suggested that NO is a mediator of MeJA-induced regulation of wheat water content during water deficit.