Abstract
Changes in intracellular free Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) in cultured hippocampal CA1 neurons isolated from newborn rats were measured by a confocal laser scanning microscope, using the Ca<sup>2+</sup> indicator Fluo-3. The results showed that exogenous adenosine (100 μM) significantly attenuated the increase of neuronal [Ca<sup>2+</sup>]<sub>i</sub> induced by acute anoxia. This effect of adenosine could be suppressed by the adenosine A1 receptor antagonist 8-cyclopentyltheophylline. Moreover, potassium channel blockers, aminopyridine, and glipizide could also block the inhibitory role of adenosine, but tetraethylammonium had no effect. These results suggest that adenosine may activate 4-AP or ATP-sensitive potassium channels via an A1-receptor-mediated mechanism and consequently inhibit anoxia-induced [Ca<sup>2+</sup>]<sub>i</sub> elevation in hippocampal neurons.
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