Involvement of inorganic elements in tissue reunion in the hypocotyl cortex of Cucumis sativus

Abstract

Cucumber (Cucumis sativus L.) hypocotyls were transversely cut to half their diameter, and morphological analyses of the tissue-reunion process in the cortex were conducted to elucidate the involvement of root-derived factors. Cell division in the cortex commenced 3 days after cutting, and the cortex was nearly fully united within 7 days. In shoots from which the roots were removed and which were cultured in water, cell division occurred during tissue reunion; however, thick-wall layer formed in the reunion region, and intrusive cell elongation and interdigitation of cortex cells at the cut surface did not occur, even after 7 days. Interdigitation of cells, followed by normal tissue reunion, was observed in shoots from which the roots were removed and which were cultured in squash xylem sap or Murashige and Skoog (MS) medium. The same effect was observed with the simultaneous application of B, Mn, and Zn, which are the major inorganic microelements of MS medium. Our results suggest that application of these inorganic elements, which are taken up from the soil and transferred to the xylem sap, are required for interdigitation of cells during tissue reunion in the cortex of cucumber hypocotyls, possibly because they are required for cell wall function and metabolism.