Abstract
To elucidate the involvement of illumination in indocyanine green (ICG) retinal toxicity. We incubated isolated rat retinas with or without illumination after exposure to 0.5% ICG. We also examined whether a time lag following ICG exposure before illumination altered the damage. Toxicity was evaluated by histologic and biochemical assays, including measurement of lactate dehydrogenase release. Retinas fixed immediately after ICG exposure showed minimal morphologic changes. However, illumination for 3 hours at 34 degrees C starting after washout of ICG selectively damaged the outer nuclear layer. Retinas incubated for 3 hours under the same condition in the dark showed preserved morphology but were damaged by subsequent illumination. When retinas were illuminated after washout of ICG at a lower temperature (30 degrees C), the damage was attenuated. Results obtained using lactate dehydrogenase release were consistent with these morphologic changes. Incubating retinas in the dark and cooling after ICG exposure significantly inhibited retinal damage, suggesting that ICG interacts with illumination to induce retinal damage.
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