Involvement of histaminergic and noradrenergic receptors in the oxytocin-induced food intake in neonatal meat-type chicks.

Abstract

Oxytocin neurons have a physiological role in food intake and energy balance. Several studies have shown that central histaminergic and adrenergic systems synapse on oxytocin neurons but there is no information for their interaction on food intake regulation in birds. The purpose of this study was to examine the effects of intracerebroventricular (ICV) injection of α-fluoromethylhistidine (α-FMH, histidine decarboxylase inhibitor), chlorpheniramine (histamine H1 receptors antagonist), famotidine (histamine H2 receptors antagonist), thioperamide (histamine H3 receptors antagonist), prazosin (α1 receptor antagonist), yohimbine (α2 receptor antagonist), metoprolol (β1 adrenergic receptor antagonist), ICI 118,551 (β2 adrenergic receptor antagonist) and SR59230R (β3 adrenergic receptor antagonist) on oxytocin-induced hypophagia in 3-h food-deprived (FD3) neonatal broiler chicken. In Experiment 1, 3h-fasted chicks were given an ICV injection of saline, α-FMH (250nmol), oxytocin (10μg) and co-injection of α-FMH+oxytocin. Experiments 2-9 were similar to experiment 1 except birds were injected with chlorpheniramine (300nmol), famotidine (82nmol), thioperamide (300nmol), prazosin (10nmol), yohimbine (13nmol), metoprolol (24nmol), ICI 118,551(5nmol) and SR59230R (20nmol) instead of α-FMH, respectively. After injection cumulative food intake was measured until 120min post injection. According to the results, ICV injection of oxytocin significantly decreased food intake in broiler chickens (P<0.001). ICV injection of α-FMH significantly attenuated hypophagic effect of oxytocin (P<0.001). Also, co-injection of chlorpheniramine plus oxytocin significantly decreased the effect of oxytocin on food intake (P<0.001). Co-administration of thioperamide and oxytocin significantly amplified hypophagic effect of oxytocin in chickens (P<0.001). In addition, ICI 118,551 attenuated hypophagic effect of oxytocin (P<0.001); while famotidine, prazosin, yohimbine, metoprolol and SR59230R had no effect on oxytocin- induced food intake in FD3 broiler chickens. These results suggest that the effect of oxytocin on food intake is probably mediated by histaminergic (via H1 and H3 receptors) and noradrenergic (via β2 receptors) systems in broiler chickens.