Abstract
Somatic embryogenesis was readily induced at a high frequency in a cell suspension culture of carrot ( Daucus carroa L. cv. «Kurodagosun») by transferring cells cultured in a medium containing auxin to an auxin-free medium after fractionation of the cells by sieving. Addition of auxin (IAA) inhibited embryogenesis at concentrations higher than 10 -7 M. Antiauxins (2,4,6-trichlorophenoxyacetic acid and p -chlorophenoxyisobutyric acid) also inhibited embryogenesis. The results of the experiments in which auxin or antiauxin was supplied at different stages of embryogenesis indicated that the early stage of embryogenesis is sensitive to both auxin and antiauxin. Presence of endogenous auxin was demonstrated in the cells at every stage of the embryogenesis by the Avena curvature test. The involvement of endogenous auxin in somatic embryogenesis is discussed.
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