Involvement of CXCL12/CXCR4 in the motility of human first-trimester endometrial epithelial cells through an autocrine mechanism by activating PI3K/AKT signaling

Jiayi Zheng,Wenhui Zhou,Danni Qu,Ling Ding,Chen Wang

doi:10.1186/s12884-020-2788-3

Abstract

BackgroundCXCL12(chemokine ligand 12, CXCL12) and its receptors CXCR4 are widely expressed in maternal-fetal interface and plays an adjust role in materno-fetal dialogue and immune tolerance during early pregnancy. This study aimed to evaluate the role and mechanism of self-derived CXCL12 in modulating the functions of human first-trimester endometrial epithelial cells (EECs) and to identify the potential protein kinase signaling pathways involved in the CXCL12/CXCR4’s effect on EECs.MethodsThe expression of CXCL12 and CXCR4 in EECs was measured by using immunohistochemistry, immunofluorescence, real-time polymerase chain reaction and enzyme-linked immunosorbent assay. The effects of EEC-conditioned medium (EEC-CM) and recombinant human CXCL12 (rhCXCL12) on EEC migration and invasion in vitro were evaluated with migration and invasion assays. In-cell western blot analysis was used to examine the phosphorylation of protein kinase B (AKT), extracellular regulated protein kinases (ERKs) and phosphatidylinositol 3-kinase (PI3K) after CXCL12 treatment.ResultsCXCL12 and CXCR4 were both expressed in human first-trimester EECs at the mRNA and protein level. Both EEC-CM and rhCXCL12 significantly increased the migration and invasion of EECs (P < 0.05), which could be blocked by neutralizing antibodies against CXCR4 (P < 0.05) or CXCL12 (P < 0.05), respectively. CXCL12 activated both PI3K/AKT and ERK1/2 signaling and CXCR4 neutralizing antibody effectively reduced CXCL12-induced phosphorylation of AKT and ERK1/2. LY294002, a PI3K-AKT inhibitor, was able to reverse the promotive effect of EEC-CM or rhCXCL12 on EEC migration and invasion.ConclusionsHuman first-trimester EECs promoted their own migration and invasion through the autocrine mechanism with CXCL12/CXCR4 axis involvement by activating PI3K/AKT signaling. This study contributes to a better understanding of the epithelium function mediated by chemokine and chemokine receptor during normal pregnancy.

Highlights

C-X-C motif chemokine ligand 12 (CXCL12)(chemokine ligand 12, CXCL12) and its receptors C-XC chemokine receptor type 4 (CXCR4) are widely expressed in maternal-fetal interface and plays an adjust role in materno-fetal dialogue and immune tolerance during early pregnancy
We used CXCR4 as control and measured CXCL12 expression in human first-trimester Endometrial epithelial cell (EEC) and explored the effects of self-derived CXCL12 on EEC invasion and migration; we explored the potential protein kinase signaling pathways involved in the effect of CXCL12/CXCR4
Red fluorescence (CXCL12) and green fluorescence (CXCR4) were both detected in these cells

Summary

Introduction

CXCL12(chemokine ligand 12, CXCL12) and its receptors CXCR4 are widely expressed in maternal-fetal interface and plays an adjust role in materno-fetal dialogue and immune tolerance during early pregnancy. This study aimed to evaluate the role and mechanism of self-derived CXCL12 in modulating the functions of human first-trimester endometrial epithelial cells (EECs) and to identify the potential protein kinase signaling pathways involved in the CXCL12/CXCR4’s effect on EECs. Successful embryo implantation and pregnancy maintenance is dependent on a receptive endometrium and close coordination between the endometrium and the conceptus [1,2,3]. Chemokine ligand 12 (CXCL12) can modulate proliferation, invasion and survival of human trophoblasts, which is crucial for the establishment of a successful pregnancy [10,11,12]. It is instructive to explore the function of CXCL12/CXCR4 in the establishment and functioning of a receptive endometrium

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