Abstract
A possibility of involvement of arachidonic acid metabolism in insulin-stimulated protein systhesis was examined in cultured L6 myocytes, myoblasts and myotubes. L6 myoblasts required ten times as high concentrations of insulin as did L6 myotubes to attain comparable stimulatory actions on protein synthesis to L6 myotubes. The insulin-induced increase in protein synthesis was interrupted by a phospholipase A2 inhibitor, mepacrine, and a cyciooxygenase inhibitor, indomethacin, in L6 myoblasts and myotubes, while the increase was not diminished at all by a lipoxygenase ihhibitor, caffeic acid, in both muscle cells. These results strongly suggest that arachidonic acid metabolite(s) produced via cyciooxygenase pathway may be involved in the signaling cascade leading from the binding of insulin and cellular receptor to protein synthesis in L6 myoblasts and myotubes.
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