Abstract
The small abalone Haliotis diversicolor is an economically important mollusk that is widely cultivated in Southern China. Gonad precocity may affect the aquaculture of small abalone. Polyamines, which are small cationic molecules essential for cellular proliferation, may affect gonadal development. Ornithine decarboxylase (ODC) and antizyme (AZ) are essential elements of a feedback circuit that regulates cellular polyamines. This paper presents the molecular cloning and characterization of AZ from small abalone. Sequence analysis showed that the cDNA sequence of H. diversicolor AZ (HdiODCAZ) consisted of two overlapping open reading frames (ORFs) and conformed to the +1 frameshift property of the frame. Thin Layer chromatography (TLC) analysis suggested that the expressed protein encoded by +1 ORF2 was the functional AZ that targets ODC to 26S proteasome degradation. The result demonstrated that the expression level of AZ was higher than that of ODC in the ovary of small abalone. In addition, the expression profiles of ODC and AZ at the different development stages of the ovary indicated that these two genes might be involved in the gonadal development of small abalone.
Highlights
Abalone is a common name of a group of marine sea snail belonging to gastropod molluscs
The overexpressed protein of AZ +1ORF2 inhibited putrescine generation. These results demonstrated that the overexpressed protein of abalone AZORF1 was the nonfunctional AZ, whereas the expressed protein of AZ +1ORF2 was the functional AZ that promoted Ornithine decarboxylase (ODC) degradation through 26S protease
The AZ approach to deplete cellular polyamine concentration has been investigated by many researchers
Summary
Abalone is a common name of a group of marine sea snail belonging to gastropod molluscs. Abalone appears earlier than mammals and lies between Coelomata and Euteleostomi. Abalone is an economically important marine shellfish that is increasingly utilized in aquaculture worldwide [1]. The small abalone H. diversicolor is distributed in the coast of South China and Japan. The aquaculture of H. diversicolor has recently encountered a series of problems, including gonad precocity and slow growth rate. The mechanism underlying the gonadal development of H. diversicolor at the cellular molecular levels is still unclear. Molecules such as gonadotropin-releasing hormone (GnRH) are reportedly
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