In-vivo Immunomodulatory Activities of Isolated Compounds from the Leaves of Amaranthus spinosus and Achyranthes aspera

Abstract

The purpose of this study was to look into the Immunomodulatory action of isolated compound of Achyranthes aspera and Amaranthus spinosus in experimental model of immunity. Cellular immunity was carried out by neutrophil adhesion test and carbon clearance assay, whereas, humoral immunity was analyzed by mice lethality test and indirect haemagglutination assay. The dose was selected after acute toxicity study of isolated compound of Achyranthes aspera and Amaranthus spinosus and administered at 5 mg/kg orally. The Levamisole (0.68mg/kg, p.o) was used as standard. Isolated compound of Achyranthes aspera (IAA) and Amaranthus spinosus (IAS) at 5 mg/kg produced significant increases in adhesion of neutrophils and an increase in phagocytic index in carbon clearance assay. Both doses of IAA and IAS significantly prevented the mortality induced by bovine Pasteurella multocida in mice. Treatment of animals with IAA, IAS and Levamisole significantly increased the circulating antibody titre in indirect haemagglunation test. Among the different Isolates, (IAS) was more effective in cellular immunity models than the (IAA). However, Both the Isolates IAA and IAS exhibited similar protection in humoral immunity procedures. It is concluded that isolated compound of Achyranthes aspera(IAA) and Amaranthus spinosus(IAS)possesses potential for augmenting immune activity by cellular and humoral mediated mechanisms more at doseof (5 mg/kg).