Abstract
Summary Chromatin isolated from chicken embryo cells, which have been shown to contain sequences of tumor virus DNA in an integrated state, was transcribed in vitro with E. coli RNA polymerase and the transcripts were assayed for the presence of viral RNA. RNA transcribed from the chromatin of cells in which viral gene functions are expressed contained readily detectable quantities of viral RNA; whereas RNA transcribed from chromatin of cells in which viral gene functions are expressed poorly or not at all contained little, if any, viral RNA. These results parallel the results obtained with RNA isolated from the two cell types. These findings indicate that the control mechanism regulating the transcription of the endogenous viral genes in chicken cells can be studied in an in vitro system utilizing isolated chromatin.
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