Invitro Study on the Combined Effects of Natural Ingredients and Antimicrobial Drugs as Novel Anti Biofilm Approach

Abstract

Biofilm forming bacteria stick to one another or to the different surfaces or interface. Biofilm formation is not a good thing in many ways as they go with low metabolic rate and passed with less number of cell divisions. Objective: To find some novel anti-biofilm approaches against biofilms. Methods: Soil and water samples were collected from four sites. Soil samples were collected from agricultural land and road side of Hudiara village, Lahore, Pakistan. However, water samples were collected from BRB canal which is situated in village Barki and from tube well of village Hudiara located in Lahore district Punjab, Pakistan. For biochemical identification of isolates different types of biochemical tests such as MR, VP, SIM (motility), H2S, catalase, Indole and nitrate reduction were performed. Results: Some antibiotics and their combinations with different other antibiotics were checked and it was noticed the overall effects of antibiotics on bacterial biofilms have positive effects except disprin and Levofloxacin. While, Ciprofloxacin was found as an effective antibiotic. Combination of ciprofloxacin and disprin was used in order to remove the biofilm and it worked well to remove the biofilm. Conclusions: Different antimicrobial medications, all-natural compounds, and combinations of various antibiotics, including ciprofloxacin-disprin, clarithromycin-moxifloxacin, and certain all-natural ingredients like honey, ginger, and lemon juice, were utilized to remove bacterial biofilms. Thus, it can be said that most of the combinations produced better biofilm removal outcomes than the individual elements did.