Abstract
This study generally aimed to determine the interaction of Chaetomium cellulolyticum with Aspergillus flavus and Curvularia lunata and the antifungal properties of Chaetomium cellulolyticum extracts against of Aspergillus flavus and Curvularia lunata. Description of the fungal microscopic association was done in slide bi-culture while the zone of colonization was studied in the co-culture method and zone of inhibition was determined in the antimicrobial bioassay. Results revealed that in slide bi-culture test, the hyphae of A. flavus were damaged when grown together with C. cellulolyticum. Co-culture method revealed that C. cellulolyticum does not colonize the agar lawn of A. flavus. In bioassay, results revealed that at 24 th to 48 th hours of incubation, mycelial crude hexane and ethanol extracts do not produced zone of inhibition on A. flavus. Statistical analysis showed that the zone of inhibition of the two crude extracts were comparable to the positive and negative control. However, at 72 nd to 96 th hours, the extracts were significantly smaller to the positive control and comparable to the negative control (p<0.05). In slide bi-culture test revealed that the hyphae and conidia of C. lunata were broken when grown together with C. cellulolyticum. In Co-culture method results revealed that C. cellulolyticum colonized the agar lawn of C. lunata by 0.15%. In Bioassay, results revealed that mycelial crude hexane and ethanol extracts do not produced zones of inhibition on C. lunata at 24 th to 96 th hours of incubation. Analysis of variance and comparison among means showed, that inhibitions of two extracts were comparable to the positive and negative control at 24 th hours of incubation. However, at 48 th to 96th hours of incubation, the crude extracts were significantly smaller to the positive control but comparable to the negative control (p<0.05). Chaetomium cellulolyticum have antibiosis mechanism to deform or damage the morphological hyphae and either conidia of A. flavus and C. lunata. However, the affectivity of crude hexane and ethanol extracts from C. cellulolyticum were not observed to inhibit the growth of A. flavus and C. lunata.
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