Invitro propagation of Fraser fir from embryonic explants

Abstract

Cotyledons, hypocotyls, and cotyledon–hypocotyl explants were excised from seeds of Fraser fir (Abiesfraseri (Pursh) Poir.) subjected to H2O2 treatment for 3, 6, 9, or 12 days and cultured on media containing 0–30 mg/L N-(phenylmethyl)-1H-purine-6-amine. Adventitious buds developed on hypocotyls and hypocotyls of cotyledon–hypocotyl explants, but rarely on cotyledons. Excised hypocotyls produced more buds than hypocotyls of cotyledon–hypocotyl expiants (1.6 vs. 1.1). Hypocotyls excised from seeds treated with H2O2 for 6 or 9 days and placed on medium containing 10 mg/L N-(phenylmethyl)-1H-purine-6-amine yielded the greatest number of buds, with a mean of three buds per hypocotyl. Elongated adventitious shoots were placed on media containing 0–40 mg/L 1H-indole-3-butyric acid to induce rooting. Forty-eight percent of shoots treated with 40 mg/L 1H-indole-3-butyric acid rooted, and the greatest number of roots per rooted shoot (3.0) was also noted at this concentration. Plantlets were transferred to a growing medium, but acclimation was unsuccessful