Abstract
Background: Biolms cause increased antimicrobial resistance and persistent infection. Aim:To detect biolm production in clinical isolates using phenotypic tests and study antibiogram. A prospective cross sectional study Method: conducted in Microbiology , KMCH IHSR. Total 94 isolates identied by standard procedures were subjected to biolm detection by Tube & Tissue Culture plate method. Antibiogram was performed by disc diffusion. The most common organism was Escherichia coli (42%) followed Results: by Klebsiella species (19%). Predominant Grampositives, were Staphylococcus aureus and Enterococcus species (36%). 6 isolates were completely resistant to antibiotics. TCP was superior to tube method. The total number of biolm production by tube and TCP method was 36(38.3%), only TCP method 46 (49%) and either by tube method or TCP method 82 (87%). Biolm producers showed higher resistance. Simple, cost-ef Conclusion: fective test may be conductedroutinely to assist surgeons / clinicians in effectively managing infections, reducing mortality and morbidity
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