Invitro assessment of wound healing mechanisms of synthesized biomaterial with spider web as a novel raw material

Abstract

The intention of the present work is to utilize the bio wastes -the pollutants that threaten the environment and to sustain the folklore practices. The wastes products of the slaughterhouse nauseate the environment and attempts are made to utilize the low value products -bovine blood and bovine collagen- to get the high value product – the synthesized wound dressing biomaterial. The topical application of the spider web and the Azadirachta indica (L.) powder on the wound had been practiced in folklore. A biomaterial was synthesized from physiologically clotted fibrin, bovine collagen, the ethanolic extract of A.indica (L.) and impregnated with spider-web as the novel raw material. The current work analyzed whether the synthesized biomaterial possessed the wound healing mechanisms or not. GC–MS analysis was done to identify the compounds present in the biomaterial. PASS software online was employed to filter off the bio active compounds possessing wound healing mechanisms. The 1,14-Tetradecanediol, 1-Octadecene, 1,E-11,Z-13-Octadecatriene, 3H– Cyclodeca[b]furan-2-one, 4,9-dihydroxy-6-methyl-3, 10-dimethylene, 9,12- Octadecadienoic acid (Z,Z)-Linoleic acid, 9,12 Octadecadienoyl chloride (Z,Z), 2H– Pyran,2-(7-heptadecynyloxy) tetra hydro, 9,12-octadecadienoic acid (Z,Z)- phenyl methyl ester, 9,12-octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl)methyl ester were the compounds found to exhibit wound healing mechanisms from a total of fifty-eight compounds identified by GC–MS analysis and further screened off by PASS software analysis. The antimicrobial activity of the biosynthesized wound dressing material was done to evaluate the potency of the biomaterial against wound susceptible micro-organisms. PASS Software online analysis of the biomaterial suggested that the bioactive compounds present in the synthesized biomaterial exhibited synergistic activities that would promote wound healing. The antibacterial activity of the biomaterial was found to be dose-dependent. The present work emphasizes the utilization of the bio wastes as bio remediation and is a step ahead for the development of an eco-friendly environment.