In-Vitro antiurolithiatic activity of aqueous extract Pavonia lasiopetala

Abstract

Background: Upto date, the scientific documentation regarding in-vitro antiurolithiatic activity of has been reported, although it has not reported for in-vitro antiurolithiatic activity up till now.
 Objective: To explore anti-urolithiatic activities of Pavonia lasiopetala leaves extract by utilizing different in-vitro models.
 Material and Methods: Aqueous extract of Pavonia lasiopetala fresh leaves was prepared and arranged in the different concentrations. Homogenous precipitation method was used to prepare artificial stones such as calcium oxalate and calcium phosphate and semi-permeable membrane of eggs was used as dissolution bags. Dissolution models were incubated in 72 hrs and after that, the entire content in dissolution bags was estimated spectrophotometrically. The inhibitory activity of Pavonia lasiopetala leaves extract on the nucleation of calcium oxalate crystals and the rate of aggregation in calcium oxalate crystals was determined by spectrophotometric assay. Results: In dissolution models, the extract of Pavonia lasiopetala has greater capability to dissolve calcium oxalate while Cystone standard has shown better demineralization for calcium phosphate rather than extract of Pavonia lasiopetala. Cystone exhibited strongly inhibitory action in the nucleation assay rather than aggregation assay. The extract of Pavonia lasiopetala exhibited inhibitory action in both of nucleation and aggregation assays to significant level. 
 Discussion: Correlation between in-vitro and in-vivo studies may be helpful to understand the molecular mechanism of litholysis process and to reveal phytochemicals of the extract responsible for dissolving or disintegrating renal calculi.
 Conclusion: Pavonia lasiopetala extract exhibited significant in-vitro anti-urolithiatic activity.
 Keywords: Pavonia lasiopetala, spectrophotometrically, in-vitro antiurolithiatic.