Abstract
INTRODUCTION:Cervical cancer is a malignant infectious disease caused by Human Papilloma Virus (HPV) in the cervix. Fig and olive oil containing flavonoid has been shown to have antioxidant and anticancer activity.OBJECTIVE:This study aimed to evaluate antioxidant and cytotoxic activity of combined Ficus carica L. and Olea europeae L. against HeLa cervical cancer cells.METHODS: This is an experimental study with a post-test only control group design. The HeLa cells were divided into 5 groups: fig extract, olive oil, combined fig extract and olive oil (at the ratio of 1:3, 1:1, 3:1), positive control (doxorubicin). The cytotoxic and antioxidant activity were evaluated by using MTT Assay and DPPH, respectively. The cytotoxic results were analyzed using probit and antioxidant activity was analyzed by using linear regression to obtain IC50 values.RESULTS:The IC50 cytotoxic of fig extract, olive oil, combined fig extract and olive oil (at the ratio of 1:3, 1:1, 3:1) with positive control (doxorubicin) were 13063,915 �g/mL, 679,593 �g/mL, 1562,356 �g/mL, 746,923�g/mL, 563,626 �g/mL and 13,707 �g/mL respectively. The IC50 antioxidant of fig extract, olive oil, and combination of fig extract and olive oil (3:1) was 105.9272 ppm, 23.1276 ppm, and 21,0689 ppm respectively.CONCLUSION:The combination of fig extract and olive oil (3:1) was shown to have the highest antioxidant and cytotoxic activity against HeLa cervical cancer cells.
Highlights
Introduction: Cervical cancer is a malignant infectious disease caused by Human Papilloma Virus (HPV) in the cervix
This study aimed to evaluate antioxidant and cytotoxic activity of combined Ficus carica L. and Olea europeae L. against HeLa cervical cancer cells
Antioxidant activity in fig extract belongs to moderate antioxidants because the IC50 value is in the range of 101-150 ppm, and the IC50 value of olive oil has antioxidant activity which is classified as very strong because it has an IC50 value of
Summary
Figs (Ficus carica L.) containing active compounds such as phenols, terpenoids, flavonoids and alkaloids in vitro have shown to have effect in inhibiting the proliferation of various cancer cells and antioxidant properties (Joseph and Justin Raj, 2011). In the study of Yao et al, (2014), it was stated that olive oil extract (Olea europaea L.) contains Oleuropein compounds and intrinsically has a cytotoxic effect on HeLa cells. Because both can potentially inhibit the proliferation of HeLa cervical cancer cells, a research related to the combination of figs and olive oil as anticancer agents is needed, so that they can be used as effective and maximum treatment and expected to have a synergistic and mutual reinforcing mechanism to inhibit proliferation of HeLa cervix cancer cell (Yao et al, 2014). Aim of the study to evaluate antioxidant and cytotoxic activity of combined Ficus carica L. and Olea europeae L. against HeLa cervical cancer cells
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