Abstract
Many herbo-mineral remedies individually or in combination have been recommended in various medical treatises for the cure of different diseases. Gandhaka Rasayana is a compound preparation, being used by Ayurvedic physicians in clinical practice for thousands of years, in the treatment of skin diseases of bacterial and fungal origin. So the drug is selected to study its anti-fungal effect on Candida albicans. Candidiasis is the most common mycotic disorder of the skin affecting the immune-compromised patients and those on the anti-biotic therapy. Hence the anti-candidal activity was examined in the study. For the study Broth Dilution method was adopted to study the Minimum Inhibitory Concentration (MIC) and Minimum Contact Time (MCT) of the Gandhaka Rasayana, following NCCLS guidelines. Results: Gandhaka rasayana showed antifungal effect against Candida albicans with Minimum inhibitory concentration (MIC) of 5.00mg/ml of Brain Heart Infusion (BHI) Broth and Minimum Contact Time (MCT) above one hour.
Highlights
After 24 hours of incubation of s dextrose agar (SDA) plates, positive control group and the Sample III were showing the growth of the Candida (Figure 6), indicating that the Candida were viable through out the experiment and the Concentration of Gandhaka Rasayana at 2.5 mg/ml of Brain Heart Infusion (BHI) Broth was not sufficient to inhibit the growth of the Candida
Minimum Contact Time (MCT): (Table 2). It was observed after 24 hours of incubation that the growth of Candida was seen till half an hour of the adding of the Gandhaka Rasayana and was absent later
This indicates that the minimum contact time (MCT) of Gandhaka Rasayana to act anti-fungally should be above one hour
Summary
Raw materials were collected from genuine sources and subjected initially for the purification process. Method: To study the antifungal activity, Invitro study by Broth Dilution Method was adopted In this five test tubes were taken and filled with 1 ml of the samples of Sample I, sample II, Sample III, Positive control group and Negative control group respectively. Procedure adopted to know the Minimum Contact Time (MCT): 5.0mg of the prepared sample (Gandhaka Rasayana) was added to 5ml of BHI broth containing Candida albicans. After 24 hours of incubation of SDA plates, positive control group and the Sample III were showing the growth of the Candida (Figure 6), indicating that the Candida were viable through out the experiment and the Concentration of Gandhaka Rasayana at 2.5 mg/ml of BHI Broth was not sufficient to inhibit the growth of the Candida. It can be said that the Minimum Inhibitory Concentration (MIC) of Gandhaka Rasayana for Candida is 5 mg/ml of BHI Broth
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