Abstract
Introduction and Aim: Herbal medicine have been used to treat several ailments since decades. Pouteria campechiana (Kunth) Baehni belongs to the family Sapotaceae which is widely found around the world. In folk medicine, various parts of P. campechiana is used to treat various illness. Inhibition of alpha-amylase and alpha-glucosidase enzymes can be an important strategy in management of postprandial blood glucose level in non-insulin dependent diabetic patient. Hence, present study focused to evaluate the in vitro antidiabetic activity of leaf and fruit methanolic extract of Pouteria campechiana (Kunth) Baehni. Materials and Methods: Methanolic extract of P. campechiana leaf (PCL) and fruit (PCF) was screened by biochemical assay such as ?-amylase inhibition activity by CNPG3 method (2-chloro-p-nitrophenyl-?-D-maltotrioside) and ?- glucosidase inhibition activity and in vitro cellular assay such as glucose uptake assay in 3T3-L1 cell line. Results: Methanolic extract of P. campechiana leaf and fruit showed inhibition of ??Amylase and ??Glucosidase enzymes. The methanolic extract of P. campechiana leaf and fruit at varying concentrations (?g/ml), did not exhibit cytotoxicity against 3T3-L1 cell line after 24 hours of incubation. The test compounds PCL and PCF induced the uptake of 2?(N?(7?Nitrobenz?2?oxa?1,3?diazol?4?yl) Amino) ? 2?Deoxyglucose (2-NBDG) in 3T3L1 cells. PCF and PCL both showed almost similar activity of standard drug, Metformin at higher concentrations. Conclusion: Based on the results, it can be concluded that methanolic leaf and fruit extract of P. campechiana possess antidiabetic activity.
Highlights
Introduction and AimHerbal medicine have been used to treat several ailments since decades
Diabetes mellitus (DM) is a metabolic disorder characterized by a chronic hyperglycemia with dysregulation of carbohydrates, fats, and protein metabolism resulting from defective secretion and function of insulin (1)
Further studies need to be carried out to understand the exact mechanism of action. This novel study reports the antidiabetic properties of P. campechiana leaf and fruit as evidenced by biochemical assays and in vitro cellular assay
Summary
Herbal medicine have been used to treat several ailments since decades. Pouteria campechiana (Kunth) Baehni belongs to the family Sapotaceae which is widely found around the world. Present study focused to evaluate the in vitro antidiabetic activity of leaf and fruit methanolic extract of Pouteria campechiana (Kunth) Baehni. Diabetes mellitus (DM) is a metabolic disorder characterized by a chronic hyperglycemia with dysregulation of carbohydrates, fats, and protein metabolism resulting from defective secretion and function of insulin (1). Diabetes mellitus is characterized by a reduced reaction of insulinsensitive peripheral tissues and a marked decrease in glucose uptake and metabolism in response to insulin. Genus Pouteria is a tropical fruit trees belonging to the family Sapotaceae. The fruit of P. campechiana is reported as a rich source for carotenoids and is well known as an antioxidant and a hepatoprotective (5)
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