Abstract

This oral presentation will describe of a new type of immunoassays (i.e., sink/float magnetic immunoassays) that could be performed in the field with a simple analytical protocol that does not include washing steps and does not need any analytical instrument (just a small permanent magnet). Sink/float magnetic immunoassays combine elements of traditional immunoassays, immunomagnetic separations, and density-based sink/float tests in a single analytical platform that can provide sensitive results with a mix and observe protocol. Sink/float magnetic immunoassays use a polymeric sphere (that is visible to bare eye) as the solid substrate of the immunoassay, detection antibodies labeled with magnetic nanoparticles, and a simple sink/float test to detect the analyte-mediated binding of magnetic nanoparticles on the polymeric sphere; if the sphere sinks, then the target analyte is present in the tested solution. In this talk the detection mechanism of sink/float magnetic immunoassays will be explained in detail. Examples of the use of sink/float magnetic immunoassays for the detection of proteins, disease-specific antibodies and bacteria will be also demonstrated.

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