(Invited) Nanozyme Integrated Microfluidic Devices for Smart Health Monitoring

Abstract

In recent years, much effort has been devoted toward developing point-of-care (POC) devices. Among them, smart phone enabled paper-based POC devices are a special category due to the advantages of being simple, rapid, on-site, and cost-effective; they have been widely used in home health care and medical testing, even environmental monitoring. The blood glucose meter is one of the most successfully commercialized diagnostic devices on the market because of its low cost, compact size, simple operation, and reliable quantitative results. However, the glucose meters are generally used for only glucose testings. Recently, many groups have reported methods establishing a direct relationship between the concentrations of the targets in the samples and the glucose detected by a glucometer, enabling a number of non-glucose biomarkers to be detected quantitatively. In this talk, we describe a novel design that combines the traditional lateral flow strip with a commercialized smartphone-enabled glucometer for portable and quantitative detection of a non-glucose target. The concept is demonstrated by using an oxidative DNA damage biomarker, 8-hydroxy-2′-deoxyguanosine (8-OHdG). The basic design of the device a colorimetric visual detection platform based on the integration of nanozyme and immunoassay. The visual detection can provide only qualitative and semiquantitative results. Thus, to enable quantitative analysis, we establish a novel method that transforms the detection of the target to the detection of an nanozyme based converting enzymatic reaction. Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events. Acknowledgement : This work was supported by NSFC NSFC 21729501 project and. the Engineering Research Centers Program of the National Science Foundation under NSF Cooperative Agreement No. EEC-1648451.