Abstract

Cytokines, acting as intercellular mediators and modulators, are essential biomolecules that regulate diverse functions within the immune response. The rapid and accurate quantification of cytokine-based immune fingerprints is vital for effectively treating immune-related diseases, particularly in point-of-care settings where immediate treatment decisions depend on precise determinations of an individual patient's immune status. Addressing the pressing need for cytokine immunoassays that offer high sensitivity, throughput, multiplexing capability, and short turnaround times at low system complexity, this presentation will discuss novel plasmonic nanomaterial-based optofluidic cytokine biosensors for immune functional analysis ranging from whole blood to single-cell levels. The multiscale experimental and theoretical research will bridge the gap in fundamental understanding of the immune system, enhancing the applicability, diagnosis, and predictive power for immune diseases. The developed platforms aim to provide biologists and clinicians with the capability to real-time monitor patients' immune statuses, a transformative achievement with immense potential for safe, effective, and personalized immunotherapy.

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