(Invited) Eight Channel Microphysiometry Using a Lab on a Chip Microclinical Analyzer

Abstract

We have built a multianalyte microphysiometer that detects multiple analytes involved in the cellular bioenergetics simultaneously. Monitoring the uptake of glucose and oxygen, and the production of lactate and acid gives a more complete picture of the metabolic processes within the cell than just acidification microphysiometry alone. Metabolic processes such as glycolysis, mitochondrial ATP generation, and glycogenesis are all directly related to the flux of these analytes. Temporal resolution of metabolic responses is much faster than conventional well-plate studies, leading to dynamic metabolic data. Electrochemical sensors are easily adapted into microfluidic formats for microphysiology readouts.Our newest instrumental designs include a complete overhaul of the microfluidic chambers, screen printed electrodes, and microfluidic pumps, valves, and drivers that will enable easy adoption of this methodology in the development of 3D cell and tissue studies using 8 separate electrochemical measurements at a time. In the first design, 8 separate amperometric sensors are developed using a screen printed electrode with isolated microfluidic channels. In the second design, 4 amperometric sensors are combined with 4 potentiometric sensors using the newly created CH1440 created by CH Instruments. The adaption of this technology to instrumenting organs-on-a-chip is currently underway.