Investigator ® Quantiplex Kit: For reliable quantification of human DNA in forensic samples

Abstract

Commonly, short tandem repeat (STR) analysis is performed for human identification, although alternative approaches, such as the analysis of deletions/insertions polymorphisms (DIPs/Indels) are now available. However, these multiplex assays used for human identification are complex and require a defined range of template input. Accurate quantification (even at low concentration) and assessment of the presence of PCR inhibitors are key to ensure successful genotyping at the first attempt. Quantitative real-time PCR is the standard method for quantification of DNA in forensic samples. However, there is a need for advanced solutions that further streamline the forensic workflow by increasing the accuracy of and reducing the time to results. The Investigator Quantiplex Kit provides fast and accurate quantification of human DNA in forensic database and casework samples. The assay provides sensitivity down to 0.3 pg/μl, with highly accurate quantification in the linear range of standard curve of 4.9 pg/μl. A balanced internal amplification control ensures detection of PCR inhibitors. The Investigator Quantiplex assay uses PCR fast-cycling technology for rapid results. When used with the Rotor-Gene Q real-time PCR system, quantification is achieved in 48 min. To further streamline the workflow and to minimize time-consuming and error-prone manual steps, it is possible to combine the Investigator Quantiplex Kit with the QIAgility instrument, a bench-top instrument allowing automation of routine procedures in the forensic laboratory workflow, such as quantification and STR setup. Combining this innovative real-time PCR-based quantification tool with liquid-handling instrumentation significantly shortens hands-on time and overall time to result, with increased accuracy and sensitivity. In addition, overall process safety is enhanced.