Investigations on the Influence of Collagen Type on Physicochemical Properties of PVP/PVA Composites Enriched with Hydroxyapatite Developed for Biomedical Applications.

Magdalena Głąb,Agnieszka Tomala,Bożena Tyliszczak,Agnieszka Sobczak-Kupiec,Anna Drabczyk,Magdalena Kędzierska,Dariusz Mierzwiński,Sonia Kudłacik-Kramarczyk

doi:10.3390/ma15010037

Magdalena Głąb, Agnieszka Tomala + Show 6 more

Open Access

https://doi.org/10.3390/ma15010037

Copy DOI

Abstract

Nowadays, a great attention is directed into development of innovative multifunctional composites which may support bone tissue regeneration. This may be achieved by combining collagen and hydroxyapatite showing bioactivity, osteoconductivity and osteoinductivity with such biocompatible polymers as polyvinylpyrrolidone (PVP) and poly(vinyl alcohol) (PVA). Here PVA/PVP-based composites modified with hydroxyapatite (HAp, 10 wt.%) and collagen (30 wt.%) were obtained via UV radiation while two types of collagen were used (fish and bovine) and crosslinking agents differing in the average molecular weight. Next, their chemical structure was characterized using Fourier transform infrared (FT-IR) spectroscopy, roughness of their surfaces was determined using a stylus contact profilometer while their wettability was evaluated by a sessile drop method followed by the measurements of their surface free energy. Subsequently, swelling properties of composites were verified in simulated physiological liquids as well as the behavior of composites in these liquids by pH measurements. It was proved that collagen-modified composites showed higher swelling ability (even 25% more) compared to unmodified ones, surface roughness, biocompatibility towards simulated physiological liquids and hydrophilicity (contact angles lower than 90°). Considering physicochemical properties of developed materials and a possibility of the preparation of their various shapes and sizes, it may be concluded that developed materials showed great application potential for biomedical use, e.g., as materials filling bone defects supporting their treatments and promoting bone tissue regeneration due to the presence of hydroxyapatite with osteoinductive and osteoconductive properties.

Highlights

The roughness and the surface topography of composite materials designed for application in implantology tissue regeneration areofone of the most important factors
It was proved that all tested composite materials showed swelling properties in the implantphysiological and the healthy tissue is a the keyhighest aspect sorption in the bone process.water
In the case of all tested materials, roughness parameter Ra was within the range 7.98–9.63 μ m, 11.0% and 8.5% lower, which was a result of the presence of numerous ions in these solutions which may increase the crosslinking density of such materials

Summary

Introduction

The development of the medical sciences taking shape over the years had a huge impact on the extension of the average human life expectancy. This is undoubtedly a great success but simultaneously a big challenge related to the necessity of the search for effective therapies of treatments of diseases that often accompany the aging of organisms including mainly diseases of the skeletal system [1]. In the case of osteoporosis, the risk of bone fractures increases significantly as a result of a decrease in their mechanical resistance [4,5]. Investigations on the development of novel bioactive composite materials that might support the treatment of bone defects and promote faster bone regeneration are very meaningful and constitute one of the biggest challenges of contemporary medicine

Objectives

Methods

Results

Conclusion