Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro

Jakov Nisavic,Nenad Milic

doi:10.2298/vetgl0606299n

Abstract

The objective of these investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the Newcastle Disease virus envelope. Six hours following inoculation of the cell line with the Newcastle Disease Vero virus, previously activated with 0.025 g/dl trypsin-versene, the appearance of hemadsorption was established in a smaller intensity in antigens diluted in ratios from 1:8 to 1:16. Hemadsorption was also determined in the mentioned cell lines 12 hours following inoculation in viruses diluted 1:64, while hemadsorption was present in virus dilutions of 1:256 after 24 hours. Inhibition of hemadsorption in inoculated cells with immune serum dilutions of 1:64 was established 24 hours following inoculation of the cell line with Vero samples of the Newcastle Disease virus, activated with trypsin-versene and the addition of specific immune serum.

Highlights

Cilj na{ih istraivanja bilo je ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spolja{njeg omota~a virusa Newcastle bolesti.
Posle 6 ~asova od inokulacije }elijske linije Vero virusom Newcastle bolesti, prethodno aktivisanim sa 0,025 g/dl tripsinversena, pojava hemadsorpcije je ustanovljena u manjem intenzitetu u razre|enjima antigena od 1:8 do 1:16.
Posle 24 ~asa od inokulacije }elijske linije Vero uzorcima virusa Newcastle bolesti, aktivisanih tripsin-versenom i dodavanja specifi~nog imunog seruma, ustanovljena je inhibicija hemadsorpcije kod inokulisanih }elija sa razre|enjem imunog seruma od 1:64.

Summary

Introduction

Cilj na{ih istraivanja bilo je ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spolja{njeg omota~a virusa Newcastle bolesti. Posle 6 ~asova od inokulacije }elijske linije Vero virusom Newcastle bolesti, prethodno aktivisanim sa 0,025 g/dl tripsinversena, pojava hemadsorpcije je ustanovljena u manjem intenzitetu u razre|enjima antigena od 1:8 do 1:16. Posle 24 ~asa od inokulacije }elijske linije Vero uzorcima virusa Newcastle bolesti, aktivisanih tripsin-versenom i dodavanja specifi~nog imunog seruma, ustanovljena je inhibicija hemadsorpcije kod inokulisanih }elija sa razre|enjem imunog seruma od 1:64.

Results

Conclusion