Investigations into the control of litter size in swine: III. A reciprocal embryo transfer study of early conceptus development2

Abstract

A reciprocal embryo transfer study with Meishan and Yorkshire pigs was conducted to examine the influence of embryonic genotype and uterine environment on preimplantation embryonic growth and development. Embryos were collected from gilts on d 2 of a spontaneous estrous cycle (d 0 = onset of estrus), transferred to synchronous recipients, and collected from recipients on d 12 to obtain measurements of embryonic diameter (size) and embryonic DNA, protein, and estrogen content. No difference was detected between Meishan and Yorkshire donors for number of ovulations, number of embryos recovered, or number of cells per embryo. Embryonic genotype affected (P < .01) d-12 embryonic characteristics; Meishan embryos (n = 101) were smaller (4.7 mm diameter) and contained less DNA (4.5 micrograms) and protein (104 micrograms) than Yorkshire embryos (n = 85; 5.9 mm, 6.1 micrograms, and 149 micrograms, respectively). Embryos (n = 80) transferred into a Meishan uterus were reduced (P < .001) in diameter (4.2 vs 6.4 mm) and in DNA (3.2 vs 7.2 micrograms), protein (103.8 vs 149 micrograms), and estrogen (352 vs 1,643 pg) content compared with embryos (n = 106) transferred into a Yorkshire uterus. These data indicate that the increased prolificacy of the Meishan breed may be due to an increased embryonic survival resulting from slower growing embryos and a suppressive effect of the uterus on embryonic growth rate and estrogen secretion.