Abstract

INVESTIGATIONS of ice crystals by means of the electron microscope have been made by several investigators1–4, but most of them were based on replication techniques. Recently, Fernandez-Moran5 reported the results obtained by low-temperature microscopy; he succeeded in stabilizing ice crystals deposited on a cold substrate by maintaining them at low temperatures during observation in the electron microscope with a microbeam of very low intensity. It was noted by him that the ice crystals were predominantly cubic and hexagonal in shape at −120° C. and at −100° to 90° C, respectively, and actually showed corresponding sharp-ring diffraction patterns.

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