Investigation on the treatment of myocardial ischemia-reperfusion injury(MIRI) with Tanyu Recipe based on integrated pharmacology platform V2.0 Molecular Mechanism Research

Abstract

Objective: Based on the integrated pharmacology platform of Chinese medicine V2.0 (TCMIP V2.0), this paper explores the molecular mechanism and quality markers of Tanyu Tongzhi Recipe against myocardial ischemia reperfusion injury (MIRI). Methods: Use TCMIP V2.0 to collect the drug components, targets, and MIRI disease targets of the recipe for phlegm and blood stasis, construct a drug-disease-target interaction network, screen drugs and disease common targets, analyze the biological process of the common targets, and finally establish a “component-target-pathway-pharmacological action” multi-dimensional network analysis and analysis of the core components in the network. Results: A total of 157 medicinal chemical components, such as Pinellia, Red Peony, Chuanxiong, Licorice, etc. were collected from the prescription of phlegm and blood stasis, with 272 corresponding targets. A total of 290 MIRI disease targets were collected. Through Traditional Chinese Medicine Association Network Mining, the first 100 targets in the core targets were analyzed and 31 targets shared by drugs and diseases. Analysis of these shared targets revealed that the shared targets are mainly involved in the positive regulation of tube formation, angiogenesis, positive regulation of autophagy, inflammation, and negative regulation of apoptosis. KEGG enrichment analysis shows that these common targets are mainly enriched in the FoxO signaling pathway, HIF-1 signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, VEGF signaling pathways and other signal pathways. Further analysis of the multi-dimensional network revealed that 46 components of Tanyu Tongzhi Prescription affect the above five signal pathways to exert anti-MIRI effects by interacting with 19 shared targets. Among them, quercetin, kaempferol, naringenin, and baicalein are intervened. AKT affects five signal pathways involved in the occurrence and development of MIRI. Conclusion: Quercetin, kaempferol, naringenin, and baicalein in Tanyu Tongzhi Recipe can resist MIRI by regulating inflammation, autophagy and apoptosis, oxidative stress, angiogenesis, and other processes.