Investigation on the antibacterial properties and rapid infected wound healing activity of silver/laterite/chitosan nanocomposites

Abstract

Infectious wounds are major threats for healthiness of human beings and challenges for clinical centers. Antibiotics cause antibiotic resistant and alternative treatments must be applied. In this work, Ag/Laterite nanocomposite (NC) was synthesized by an eco-friendly method. The as-synthesized NC was coated with chitosan (Ag/Ltt/Chn-NCs) for healing the infectious wounds. The physicochemical properties of the Ag/Ltt and Ag/Ltt/Chn-NCs were characterized using XRD, FESEM, elemental mapping, EDX, TEM, FT-IR, and DLS techniques. Based on the TEM image, synthesized spherical Ag nanoparticles were formed on the sheet-like structure of laterite, ranging from 12 to 26 nm. In addition, according to EDX, the presence of carbon, oxygen, silicon, aluminum, ferrous, and silver elements was confirmed in the as-synthesized Ag/Ltt-NCs. In vitro antibacterial properties were investigated and tissue bacterial count, pathological parameters, and gene expression were assessed. The results indicated the antibacterial properties of Ag/Ltt and Ag/Ltt/Chn-NCs at in-vitro and in vivo antibacterial activity condition; that this property leads to increase the collagen synthesis and re-epithelization and accelerated the wound healing process through involvement in the expression of pro-inflammatory, TGF-β1, and bFGF gens. Overall, Ag/Ltt/Chn-NCs are safe structures for accelerating infected wound healing and could be utilized in clinical condition after future studies.