Abstract
Baizhu Shaoyao San (BSS) is a crucial traditional Chinese medicinal formula widely applied for the treatment of painful diarrhea, diarrhea-predominant irritable bowel syndrome, ulcerative colitis, and some other gastrointestinal diseases. Corresponding to the clinical medication, the three medicinal herbs (Atractylodis Macrocephalae Rhizoma, Paeoniae Radix Alba, and Citri Reticulatae Pericarpium) included in BSS should be processed using some specific methods of stir-frying. To find the underlying correlations between serum chemical profiles and curative effects of crude and processed BSS on ulcerative colitis rats, and further explore for the effective material basis of processing, an UHPLC/Q-TOF-MS/MS technique coupled with gray correlation analysis (GCA) was developed. A total of 134 compounds were identified in rat sera after oral administration of BSS, among which 24 compounds were prototypes and 110 compounds were metabolites. Meanwhile, an ulcerative colitis model was established in rats by enema with 2,4,6-trinitrobenzene sulfonic acid, and the pharmacodynamic indicators for drug efficacies were evaluated as well. According to the results, processed BSS showed better efficacy than crude BSS. The top 10 potential effective components with high degree of correlation were identified based on GCA results, which were thought to be the crucial compounds that contributed to the enhancement of therapeutic effects in BSS after processing.
Highlights
IntroductionMolecules 2019, 24, 940; doi:10.3390/molecules24050940 www.mdpi.com/journal/molecules (Ranunculaceae), Citri Reticulatae Pericarpium (Rutaceae), and Saposhnikoviae Radix (Umbelliferae) at a ratio of 6:4:3:4
As a famous and classic herbal formula in traditional Chinese medicine (TCM), Baizhu ShaoyaoSan (BSS) is composed of Atractylodis Macrocephalae Rhizoma (Asteraceae), Paeoniae Radix AlbaMolecules 2019, 24, 940; doi:10.3390/molecules24050940 www.mdpi.com/journal/molecules (Ranunculaceae), Citri Reticulatae Pericarpium (Rutaceae), and Saposhnikoviae Radix (Umbelliferae) at a ratio of 6:4:3:4
Current studies are mainly focused on the TCM processing effect on individual herbs, and few researchers have examined the effects of processing on Chinese herbal formulas, which could result in inconsistency with clinical medication practice [9,10]
Summary
Molecules 2019, 24, 940; doi:10.3390/molecules24050940 www.mdpi.com/journal/molecules (Ranunculaceae), Citri Reticulatae Pericarpium (Rutaceae), and Saposhnikoviae Radix (Umbelliferae) at a ratio of 6:4:3:4 It was first recorded in “Dan-Xi-Xin-Fa”, a comprehensive medical treatise edited by distinguished physician Zhenheng Zhu (1281–1358 A.D.) in the Yuan Dynasty, to be commonly used as a mediative formula for softening liver and tonifying spleen, as well as eliminating dampness and alleviating diarrhea [1]. The efficacies of BSS are the synergistic results of some bioactive substances from each medicinal herb contained in the formula, such as sesquiterpene lactones in Atractylodis Macrocephalae Rhizoma, monoterpene glycosides in Paeoniae Radix Alba, flavonoids and flavonoid glycosides in Citri Reticulatae Pericarpium, and coumarins and chromones in Saposhnikoviae Radix, which reveal the so-called prescription compatibility (fufang peiwu) characteristics of TCM [4,5]. Atractylodis Macrocephalae Rhizoma should be stir-fried using honey-processing wheat bran, Paeoniae Radix Alba should be stir-fried using wheat bran, and Citri
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