Investigation on Neuroprotective Potential of Proteins Extracted from Leaves of Orthosiphon stamineus

Abstract

The key hallmarks in most of the central nervous system disorders are neurotoxicity and neurodegeneration. Neuroprotection has emerged as an alternative treatment strategy. Orthosiphon stamineus (OS; cat’s whiskers; “misai kucing”) is a medicinal plant (Lamiaceae family) with its proteins remain unexplored. This study investigates the neuroprotective potential of proteins extracted from OS leaves (OSLP) on human SH-SY5Y cells and adult zebrafish (Danio rerio). The findings obtained provide new insights into the protein extraction method from OS leaf sample using OTM achievable at laboratory scale, the neuroprotective potential of OSLP against H2O2 stress, and the anticonvulsive potential of OSLP under a normal condition and a hypoxic-ischemic condition.