Abstract
The main attention of present work is to study the molecular level interactions in the interface of biocomposite to increase their applicability. A specific kind of molecular interaction namely, hydrogen bonds play a vital role in deciding composite property. In this study, we construct a tri-component system based on silk fibroin/sodium alginate/hydroxyapatite by varying protein and polysaccharide proportions using in-situ co-precipitation method. The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite. During composite preparation, conformational changes from the random coil to β-sheet structure through intermediate β-turns exist within the protein molecule that is confirmed by vibrational spectra. The crystallographic profile and morphology of HAP were greatly influenced by virtue of polymer matrix. Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein. Moreover, the in-vitro biomineralization, cytotoxicity and antibacterial activity of composite were analysed in detail.
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