Investigation on μ-opioid receptor in Sera of Iraqi Male addiction Tramadol or Methamphetamine

Abstract

In Iraq, Drug addiction especially on Methamphetamine, (Meth); common name Crystal, and Tramadol (Tra) has increased after the year 2003. It becomes a dangerous issue, due to their multi dangerous negative effects on the health, economic, social for human, finally, it causing death. The aim of the present study is too sought out and to investigate the μ-opioid receptor (MOR) in Sera of Iraqi Male Addiction Tra or Meth. To do this, the work enrolled on 180 heavy smokers Iraqi male individuals at aged range 15-43 years (from January 2018 to December 2018) they were classified to 3 groups: G1 who were healthy control; G2 who was addicted on Meth with a dose ranged (1 - 5.0 gm for duration 1 -5 years); G3 who were addicted on Tra with an average dose (2 - 5.0 g) for duration 1 -5 years. The addiction individuals were admitted to Ibn-Rashid Hospital in Baghdad city to get the treatment. The MOR Concentration was determined by ELISA Technique while the drug level in the serum was determined by High Performance Liquid Chromatography (HPLC). The results showed a highly significant decrease (p<0.0001) in the level of MOR of the two addicted groups in comparison with the healthy group, especially those who addicted to Meth more than the others who addicted to Tra. Also the results also showed a strong negative correlation between MOR and dose (r = - 0.9022, - 0.8989) and duration of addiction (r = - 0.8989, - 0.8809) the serum of G2, G3 from the above results, The biochemical factor MOR can be used as a good marker to identify and follow up the addicted person.