Abstract
A voltammetric enzyme-linked immunoassay based on a new system of o-aminophenol (OAP)-H2O2-horseradish peroxidase (HRP) has been developed and used for detection of HRP, labelled HRP and southern bean mosaic virus (SBMV). HRP or labelled HRP catalyzes the oxidation reaction of OAP with H2O2, the product of which produces a sensitive voltammetric peak at a potential of –0.43 V (vs. SCE) in Britton-Robinson (BR) buffer solution. By using this voltammetric peak, HRP can be measured with a detection limit of 6.0×10–13g/mL and a linear range of 1.0×10–12–4.0×10–9 g/mL. The southern bean mosaic virus (SBMV) has successfully been detected, and satisfactory results have been obtained.
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