Abstract
In the development of bioartificial liver support, superior efficacy, safety, and easy-operation are required. In these viewpoints, we compared several kinds of perfusion method and hepatic function unit, and determened the most desirable combination. We have established a method of xenogeneic direct hemoperfusion of bioartificial liver support, consisting of a leukocyte adsorbent column, an immunoglobulin adsorbent column, and a hepatic function unit1. We applied this method for hybrid bioartificial liver system containing a bioreactor filled with porcine hepatocytes immobilized on nonwoven polyester fabric (NWF bioreactor), and then an extrcorporeal whole swine liver system2 in experiments of canine liver failure model. In these experiments, fluent perfusion without hyperacute rejection, and adequate ammonia detoxification was exhibited. In the further step, we utilized cross plasma perfusion (CPP) method for the NWF bioreactor, whole swine liver, and hepatocyte perfusion systems3. The whole swine liver system revealed best efficacy, but showed difficulty of maintenance. The hepatocyte perfusion systems can be prepared quickly and easily, but the efficacy is inferior to others because of quantity limit of hepatocyte. We concluded that the best functional performance is given by whole swine liver system, but considering the balance of efficacy, safety, and easy-operation, the hybrid system in CPP method is most desirable. 1. International Patent Application for PCT: No. PCT/JPO1/02233. 2000.3.22 2. Domestic Patent Application: 2001–401200. 2001.12.28 3. Domestic Patent Application: 2002–236760. 2002.8.15
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