Investigation of Thuja orientalis Leaves Extract's Anti-Arthritic Activity in Freund's Complete Adjuvant-Induced Arthritis in Wistar Rats

Abstract

A severe auto-immune disease that destroys the body's joints and may render a person physically incapable is rheumatoid arthritis. In Ayurveda, herbs have been used to cure a wide range of illnesses. Herbal medicines are widely available and have fewer side effects. As a result, many people find herbal treatments to be intriguing. The Thuja orientalis is a valuable plant for Chinese and Indian medicine. The leaves of Thuja orientalis have long been used in traditional medicine to treat rheumatoid arthritis (RA). However, The current study investigates the anti-arthritic mechanism and phytochemical evaluations of methanolic extract of Thuja orientalis leaves. Researchers employed rats that had Freund's Complete Adjuvant (FCA)-induced arthritis to test the anti-arthritic potential of methanol extracts from Thuja orientalis. Among other things, we have examined paw edoema, body mass index, locomotor activity, haematological observations, biochemical assessment, arthritic score, radiography, etc. Rats were given 0.05ml of FCA subcutaneously to cause arthritis. Four groups of arthritic rats and one group of normal rats, each with six animals, were created. The arthritic rats in Groups IV and V got 200mg per kg and 400mg per kg of thuja orientalis extract, respectively. Diclofenac sodium (10mg per kg) was given as a routine medication to Groups III of the arthritic rats, respectively, and CMC (1ml/kg) was given to Group I of the normal animals. The disease control groups were Group II respectively. Paw volumes were measured using a plethysmometer at 1, 7, 14, 21 days. When compared to arthritis control rats, the Thuja orientalis extract (200 and 400mg/kg orally) significantly (p<0.05, p<0.01) decreased the size of the paws and changed the body weight in CFA rats at day 21. In statistical analysis, all data were reported as mean SEM. The mean values of the test groups and the control group were compared using one-way ANOVA and Dunnett's test. The results of the current investigation showed that a methanol extract from Thuja orientalis leaves may have anti-arthritic properties.