Investigation of the relationship of latent tuberculosis infection with the development of myeloid and lymphoblastic leukemia

Abstract

Annotation. The relationship between tuberculosis infection and hemoblastosis has attracted the attention of physicians and has been the basis for a wide variety of assumptions. In the second half of the XIX century, there was expressed an opinion about the unique nature of these pathologies. They explained this phenomenon as the immunochemical affinity of specific tumor and mycobacterial antigens, but not as a result of the persistence of mycobacterium tuberculosis (MBT). The aim of the study was to investigate the association of latent tuberculosis infection and oncogenesis, in particular with leukemias. From transplantable human myeloblasts with acute myeloid leukemia (Kasumi-1) and T-lymphocytes with T-lymphoblastic leukemia (Jurkat), including 0.22 μm filtrate of their lysates were isolated from mycobacterium tuberculosis (MBT) with deficient cell wall (CWD). During primary microscopy, the MBT was found mainly in the form of coccoid. The isolated coccoid Kasumi and Jurkat had close antigen affinity and shared antigens with typical MBT. After prolonged incubation with the growth promoter, not only CWD MBT was isolated from all lysates and filtrates, but isolates with identical morphology and antigenic composition were obtained. This strongly suggested that the MBT and their modified forms could be associated with various types of leukemia. Moreover, the affinity of FLK-BLV isolates with blood of people with latent tuberculosis infection, cattle tuberculosis, CWD forms M. bovis and M. tuberculosis was determined, confirming the role of tuberculosis infection in the development of various forms of the disease depending on the host genome, as well as the features of the adaptive reactions of the infectious agent. In particular, after destruction by ultrasound and filtration through Amicon Ultracel® 100 K and Millex® GP 0.22 μm, their filtrates gave an increase in the number of CWD MBT with acid-resistant elements and had the same antigenic composition, including the original isolates. The long-term incubation of the test material in the growth promoter and numerous transplants on the adapted MyCel DW medium played a major role in the allocation of CWD.